introduction and history

introduction and history

The Center for Advanced Microscopy and Imaging (CAMI) is an all-University research, teaching and service facility located in Pearson Hall on Miami University's main campus, in Oxford, Ohio. Serving the microscopy needs of both the biological and materials communities.

This web site is designed as a resource for all interested students, faculty, staff and new, current, and future users of the lab.

The microscopy facility was established in 1987 as a shared facility through continued funding from the College of Arts and Science, the Graduate School, and a Botany Department Academic Challenge Grant. In 1996 the Electron Microscopy Facility (EMF) was administratively centralized under the College of Arts and Science. As the EMF diversified in both the disciplines it served and the types of microscopy it supported, the title of "Electron Microscopy" was no longer appropriate, and the Facility was renamed as the Center for Advanced Microscopy and Imaging. CAMI provides instruction and consultation in transmission and scanning electron microscopy, light microscopy, and digital imaging for undergraduates, graduates, post-docs, faculty, and staff. CAMI is used by members of the Departments of Anthropology, Botany, Chemistry and Biochemistry, Engineering Technology, Geology, Mechanical & Manufacturing Engineering, Microbiology, Paper Science and Chemical Engineering , Physics, Speech Pathology and Audiology, and Zoology, as well as by researchers from a number of regional Colleges and Universities. CAMI is available for use without charge to the Miami University community.

CAMI houses two scanning electron microscopes (JEOL 840a and Zeiss Supra 35VP), three transmission electron microscopes (JEOL 1200, JEOL 2100 and Zeiss 10C), three laser scanning confocal microscopes (Olympus FV500, Nikon PCM 2000, and Zeiss 710), a deconvolution light microscope (Olympus IX-81), a wide-field multimode light microscope (Olympus AX-70), and a fluorescence stereoscope system (Olympus SZX-12). Both SEM'sare equipped with an energy-dispersive x-ray microanalysis (EDAX EDAM & Genesis 2000), one SEM has an electron back scattered diffraction system (HKL EBSD). The JEOL 2100 STEM has energy-dispersive x-ray microanalysis (Bruker), electron energy loss spectroscopy (Gatan EELS) and energy selective imaging (ESI Gatan GIF). One confocal is mounted horizontally (Nikon PCM 2000) and one confocal has full spectral imaging (Zeiss 710). All electron microscopes are covered under service contracts.

CAMI has a diversity of computer and digital imaging/analysis capabilities. The Facility is hard wired with category 6 ethernet which provides for its own local area gigabit network as well as providing access throughout the Miami University network and the Internet. Digital images can be captured from a diversity of input sources including direct digitizing from the microscopes, video cameras, VCRs, copystand dSLR cameras, and high resolution film and flatbed scanners (prints and negatives). Image manipulation/analysis software includes: Image Pro Plus, Global Lab Imaging, Adobe Photoshop, Corel Presentations, Corel Photopaint, and others. Images can be output via high resolution and wide-format printers ( Epson 9600, Lexmark Optra Rn+ laser printer, Kodak 8650PS dye sublimation printer, Mitsubishi CP700 dye sublimation printer, Epson stylus photo ink jet printers, Polaroid 7000 Film recorder). Data storage is handled via 4 TB of on-line storage, DVD or CD-ROM recording.

In addition to being fully equipped for conventional specimen preparations, CAMI is specialized for cryo-preservation techniques. CAMI houses one of the few high pressure freezing units in North America (Balzers HFP 010), a propane jet freezing unit (RMC MF7200), a slam freezer (Pelco "Gentleman Jim"), and an automated freeze-substitution system (Reichert CSAuto). In addition, the Facility has 26+ pieces of specimen preparation equipment and 4 fully-equipped darkrooms. A small library of Journals and books is also available.

CAMI occupies 28 rooms with approximately 5400 square feet of laboratory and lecture space. This space was new renovated in 2010, and designed specifically to best meet the environmental needs for advanced microscopy. This renovation was also designed to provide the best integrated teaching and research space, and contains an interactive multi-media teaching/lecture lab for remote operation of the microscopes. All the laboratory rooms are climate controlled and the TEM lab spaces have building independent humidity controls.

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richard e. edelmann, ph.d.,
director
matt l. duley, m.s.,
microscopy specialist
questions? comments?
edelmare@miamioh.edu
9 upham hall
513.529.5712


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